THE ROLE OF CYTOLOGICAL METHOD IN THE DIAGNOSIS OF PARAIMPLANT INFLAMMATION AFTER PRIMARY TOTAL KNEE REPLACEMENT Galashina

Aims. The aim of the study was to investigate the feasibility of cytological method in the diagnosis of paraimplant inflammation after primary total knee replacement. Materials and methods. Cytological punctate preparations and impression smears of para-articular tissue of the knee endoprosthesis area were studied in 20 patients with primary instability of the endoprosthesis components. Aseptic instability was found in 11 patients, and septic instability found in 9. The cell composition of fluid samples and impression smears were studied by counting of at least 100 cells in several fields of view using the immersion lens. Results. Cytogramme of the punctates of patients with aseptic instability of the knee joint characterised by the absence of cellular inflammation elements and fibrotic cells, single lymphocytes and synoviocytes were encountered in the impression smears. Two types of cytogrammes were found in patients with septic instability of the knee joint, characterised by changing ratios of the cellular inflammation elements and acute and subacute inflammation consistent with the picture. Conclusions. Cytological method is an available, highly effective study that can be used for examination of punctates and impression smears of para-articular tissues of knee endoprosthesis area.


INTRODUCTION.
Knee arthrosis is a common disease associated with pain and disability. In 7.5% of all cases, patients aged >55 suffer knee pain, where X-ray radiography confirms gonarthrosis; in 2% of all cases, pain is coupled with motor disorders due to the limited function, which is a serious medical, social, and economic concern [1]. Knee replacement is currently one of the most efficient surgeries that helps restore the joint function and improve the quality of the patient's life [2,3]. Like any surgery, knee replacement might be followed by a variety of complications, such as paraprosthetic fractures or primary aseptic loosening [4,5]. However, periprosthetic infection is the gravest complication of primary replacement; its occurrence rate is 0.3% to 1.5% [6,7]. Early diagnosis is difficult due to the limited sensitivity and specificity of the existing biochemical, serological, and microbiological markers, especially when the infectious inflammation is subclinical and latent. The existing clinical laboratory tests mainly reveal the systemic body response to the infection; however, they cannot describe the locus of infection in the knee. This is why finding a simple and informative method for diagnosing latent periprosthetic infection is an urgent issue. To that end, clinicians use the cytological diagnosis of periprosthetic infection, which helps effectively differentiate the type of inflammation, the specific cellular reactivity, and the duration of inflammation as a typical pathological process [8,9].
Russian authors have recently published the findings of a number of studies, where they cytologically tested the liquid produced by tapping the paraarticular tissue in major joints [10].
The goal hereof is to study the feasibility of cytological testing for paraimplant inflammation after primary knee replacement.

MATERIALS AND METHODS.
In 2018, Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery, Saratov State Medical University n.a. V.I. Razumovsky, the Ministry of Health of the Russian Federation had 20 patients with the primary loosening of the knee replacement components: aseptic in 11 (55%) and septic in 9 (45%) cases. Patients were aged 60±7.5 on average, 16 women, 4 men.
The study protocol followed guidelines for experimental investigation with human subjects in accordance with the Declaration of Helsinki and was approved by the ethics committee. Written informed consent was obtained from each patient (or official representative) before the study.
Cytological tests were performed on the liquid extracted by paracentesis from the neoplasms around the implant before surgeries, as well as on the smears taken off the paraarticular knee tissue during surgeries. The joint aspirate was centrifuged at 2000 rpm over 10 minutes to obtain the sediment, from which the cytological preparations were made, 5 per patient. Joint aspirate sediments and soft tissues were applied to defatted slides. Leukodif 200 set was used for fixing and staining. The cellular composition was identified by counting at least 100 cells using a Revelation III SkyOptic microscope and a 10×100 immersive lens.

RESULTS
Notably, the median macrophage count was 5 cells (p2=6.05×10 -8 ) higher than in acuteinflammation cytograms and 6 cells higher (p3=3,29×10 -14 ) than in aseptic-loosening cytograms; the median histiocyte count was 7.5 (p2=6.05×10 -8 ) higher than in the case of acute inflammation and 8.5 higher than (p3= 3.29×10 -14 ) in the case of aseptic loosening (p3= 3.29×10 -14 ). The data contain median values (Me), lower quartiles and upper quartiles (25%; 75%); p is the two-sided significance score; p1 is the significance score, acute inflammation vs aseptic loosening; p1 is the significance score, subacute inflammation vs acute inflammation; p1 is the significance score, subacute inflammation vs aseptic loosening DISCUSSION. The cytological samples of patients with aseptic loosening contained singular synovial cells, a sign of a preserved sufficiently large pool of metabolically active synovium, which is a lining of paraarticular tissue, in which the cellular reactivity is low when there is no inflammation; it is triggered by the particles that appear due to the wear of the friction couples, as well as due to the varying mechanical impact of materials on the residual bone structures and soft tissue.

Cellular inflammation signs in patients with
In the acute-inflammation cytogram in patients with septic knee loosening, neutrophil leukocytes were prevalent, which might be a sign of a protective response to an infectious agent; or a sign of inflammatory activity even if the pathogen cannot be isolated for any reason. Macrophages and histiocytes present in such cytograms were indicative of the inflammation site being confined or the inflammation process becoming chronic [10].
The subacute inflammation cytograms sampled from these patients had greater lymphocyte counts, a sign of proliferation and recovery in the damaged tissue, while lower neutrophil leukocyte counts indicated an active arrest of paraimplant inflammation. Higher macrophage and histiocyte counts indicated a less active subacute inflammation [11].

1.
Cytological testing is an affordable and efficient technique for examining the paraarticular tissue sampled around a knee implant.

2.
In patients with aseptic loosening of their knee implants, cytological preparations did not show any markers of inflammation, whereas synoviocytes indicated zero inflammation in the paraarticular tissue.

3.
Patients with septic loosening had two types of cytograms: acute and subacute inflammation.

FINANCIAL SUPPORT AND SPONSORSHIP
Nil.